Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MicroRNA-16 inhibits the migration and invasion of glioma cell by targeting Bcl-2 gene

Baochang Luo¹, Jing Zhang²

¹Department of Neurosurgery,; ²Department of Clinical Laboratory, Hanchuan People’s Hospital, Hanchuan City, Hubei Province, China.

For correspondence:- Jing Zhang Email: ejkbv6@163.com

Accepted: 2 December 2020 Published: 29 December 2020

Citation: Luo B, Zhang J. MicroRNA-16 inhibits the migration and invasion of glioma cell by targeting Bcl-2 gene. Trop J Pharm Res 2020; 19(12):2499-2504 doi: 10.4314/tjpr.v19i12.3

© 2020 The authors.
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Abstract

Purpose: To investigate the effect of microRNA-16 (miR-16) on glioma cell migration and invasiveness, and the mechanism involved.

Methods: MicroRNA-16 mimic or inhibitor was transfected into human glioma (SHG44) cells. Cell migration, invasiveness and morphology were determined using scratch test, Transwell invasion assay, and immunohistochemical staining, respectively. expressions of bcl-2, MMP-9 and MMP-2, and NF-κB1 proteins were measured using Western blotting.

Results: Overexpression of MicroRNA-16 significantly down-regulated MMP-9 protein in SHG44 cells (p < 0.05), but MMP-2 protein expressions in the 2 groups were comparable (p > 0.05). Protein expressions of MMP-9 and NF-κB1 were significantly down-regulated in human glioma positive cells, relative to negative control.

Conclusion: MiR-16 overexpression suppresses the migration and invasiveness of SHG44 cells via the regulation of NF-κB1/MMP-9 signaling pathway, and it directly targets bcl-2 gene by inhibiting its protein expression. This finding affords a new target for developing new anti-glioma drugs.

Keywords: Bcl-2, expression, Glioma, MicroRNA-16, NF-?B1signaling pathway